Phosphorylated transcription factor PuHB40 mediates ROS-dependent anthocyanin biosynthesis in pear exposed to high light (2024)

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Lu Zhang

College of Agriculture and Biotechnology, Zhejiang University

,

Hangzhou 310058, Zhejiang

,

China

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Lu Wang

College of Agriculture and Biotechnology, Zhejiang University

,

Hangzhou 310058, Zhejiang

,

China

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Yongchen Fang

College of Agriculture and Biotechnology, Zhejiang University

,

Hangzhou 310058, Zhejiang

,

China

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Yuhao Gao

College of Agriculture and Biotechnology, Zhejiang University

,

Hangzhou 310058, Zhejiang

,

China

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Jun Su

Institute of Horticulture, Yunnan Academy of Agricultural Sciences

,

Kunming 650205

,

China

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Junbei Ni

College of Agriculture and Biotechnology, Zhejiang University

,

Hangzhou 310058, Zhejiang

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China

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Yuanwen Teng

College of Agriculture and Biotechnology, Zhejiang University

,

Hangzhou 310058, Zhejiang

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China

Hainan Institute of Zhejiang University

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Sanya, Hainan 572000

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China

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Songling Bai

College of Agriculture and Biotechnology, Zhejiang University

,

Hangzhou 310058, Zhejiang

,

China

Corresponding author: Songling Bai (songlingbai@zju.edu.cn)

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Published:

06 June 2024

Article history

Received:

22 December 2023

Revision received:

15 May 2024

Accepted:

18 May 2024

Published:

06 June 2024

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Abstract

Plants are increasingly vulnerable to environmental stresses because of global warming and climate change. Stress-induced reactive oxygen species (ROS) accumulation results in plant cell damage and even cell death. Anthocyanins are important antioxidants that scavenge ROS to maintain ROS homeostasis. However, the mechanism underlying ROS-induced anthocyanin accumulation is unclear. In this study, we determined that the HD-Zip I family member transcription factor PuHB40 mediates ROS-dependent anthocyanin biosynthesis under high-light stress in pear (Pyrus ussuriensis). Specifically, PuHB40 induces the PuMYB123-like–PubHLH3 transcription factor complex for anthocyanin biosynthesis. PuHB40-mediated transcriptional activation depends on its phosphorylation level, which is regulated by protein phosphatase PP2A. Elevated ROS content maintains high PuHB40 phosphorylation levels, while also enhancing PuHB40-induced PuMYB123-like transcription by decreasing PuPP2AA2 expression, ultimately leading to increased anthocyanin biosynthesis. Our study reveals a pathway regulating ROS-induced anthocyanin biosynthesis in pear, further clarifying the mechanism underlying abiotic stress-induced anthocyanin biosynthesis, which may have implications for improving plant stress tolerance.

Phosphorylated transcription factor PuHB40 mediates ROS-dependent anthocyanin biosynthesis in pear exposed to high light (11) Accepted manuscripts

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